N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5)

NDRG1 (N-myc downstream-regulated gene 1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG1 family comprised of four members (NDRG1-4) that function in growth, differentiation and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5) and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL),\nwhich is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDGR1 and NDGR2 are substrates for SGK1 although the precise physiological role of this phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by\nSGK1 primes NDRG1 for phosphorylation by GSK3. Phosphorylation at Thr346 was discovered using an Akt substrate antibody and was shown to be induced by insulin treatment in multiple cell lines.\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:800\nFlow Cytometry: 1:200\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X\nTBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunohistochemistry (Paraffin): 1:500\nImmunocytochemistry: 1:800\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2915-57

Size

100ul

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Shimono, A. et al. (1999) Mech Dev 83, 39-52. (2) Zhou, D. et al. (1998) Cancer Res 58, 2182-9.\n(3) van Belzen, N. et al. (1997) Lab Invest 77, 85-92 (4) Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44. (5) Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8. (6) Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105. (7) Stein, S. et al. (2004) J Biol Chem 279, 48930-40. (8) Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42. (9) Nishio, S. et al. (2008) Cancer Lett 264, 36-43.\n(10) Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58. (11) Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56. (12) Taketomi, Y. et al. (2007) J Immunol 178, 7042-53. (13) Murray, J.T. et al. (2004) Biochem J 384, 477-88.

Additional Info

Recognizes NDRG1, phosphorylated at (Thr346). Species Crossreactivity: human, monkey, mouse, rat.

Alternative Names

N-myc Downstream Regulated 1