Home  >  Products  >  N4BP1 (Y415) pAb
N4BP1 (Y415) pAb

N4BP1 (Y415) pAb

Cat no: BS3340

Supplier: Bioworld Technology, Inc.
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73(alpha), a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73(alpha) for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73(alpha) and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
Catalogue number: BS3340
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: O75113
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 391-440 of Human N4BP1.
Species: N4BP1 (Y415) pAb detects endogenous levels of N4BP1 protein.
Molecular weight: ~100 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: NEDD4 binding protein 1; N4BP1; KIAA0615
Additional info: For research use only, not for use in diagnostic procedure.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
Bioworld Technology, Inc.
Get a Quote Direct from
Bioworld Technology, Inc.

By submitting this form you agree to your details being passed to Bioworld Technology, Inc. for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave