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Na+/K+-ATPase (alpha)1 (A254) pAb

Na+/K+-ATPase (alpha)1 (A254) pAb

Cat no: BS3190

Supplier: Bioworld Technology, Inc.
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The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, (alpha), (beta) and (gamma), comprise to form the Na+/K+-ATPase oligomer. The (alpha) subunit contains the binding sites for ATP and the cations; the glycosylated (beta) subunit ensures correct folding and membrane insertion of the (alpha) subunits. The small (gamma) subunit co-localizes with the (alpha) subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP.
Catalogue number: BS3190
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: P06685
Dilutions: WB: 1:500~1:1000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 221-270 of Human Na+/K+-ATPase (alpha)1.
Species: Na+/K+-ATPase (alpha)1 (A254) pAb detects endogenous levels of Na+/K+-ATPase (alpha)1 protein.
Molecular weight: ~100 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: A X actin like protein; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB; Actin cytoplasmic 1
Additional info: For research use only, not for use in diagnostic procedure.

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