NaDC-1 (Sodium Dicarboxylate Transporter)

The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity. Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys. The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues. These are NADC1and NADC3 (NADC2 is found in C. Elegans). NADC1 is Na+-coupled, electrogenic, and exhibits low affinity for its dicarboxylatesubstrates. This isoform is expressedprimarily in the brush border membrane of intestinal and renalepithelial cells. The physiological function of NADC1 is to absorbthe intermediates of the citric acid cycle, such as citrate, succinate,-ketoglutarate, fumarate, and malate, in the intestine and kidney.NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibitsrelatively higher affinity for its substrates compared with NADC1. The NADC3is expressed primarily in the basolateral membrane of intestinaland renal epithelial cells. However, it is also found in tissuessuch as liver, placenta, and brain.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence technique.\nELISA: 0.5-1ug/ml; N0015E Control peptide can be used to coat ELISA plates at 1ug/ml. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N0015D

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.5, 0.05% sodium azide, 0.2% BSA 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1Mann, S. S et al (1999) Cytogenet. Cell Genet. 84: 89-90; 2. Pajor, A. M (1996) Am. J. Physiology, 270: F642-48; 3. Rogina, B et al (2000) Science 290: 2137-2140, 4. Wang, H et al (2000) J. Physiol Cell Physiol 278: C1019-1030.

Additional Info

Recognizes human NaDC-1. Species Sequence Homology: rabbit - 66%; mouse - 64%; rat - 57%.