NBC3, Human, Control Peptide (HCO3 Cotransporters 3)

A 16-aa peptide sequence within the cytoplasmic, N-terminus of human NBC3.\n\nBicarbonate, along with CO2, is the major pH buffer of biological fluids. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+-exchanger 3 and Na+-H+-ATPase with the basolateral Na+-HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.\n\nNBC3 (mouse 1089 aa, human 1044 aa) has 3 three transcripts, which are expressed in a tissue specific manner (9 kb in brain, placenta, lung, liver, muscle, kidney, pancreas, stomach, thyroid, and adrenal etc). NBC3 has two variants. NBC3 is 56% identical with NBC1 and 76% with NBC2.\n\nApplications:\nSuitable for use in ELISA. Not suitable for Western Blot due to low MW: <3kD. Other applications not tested.\n\nReccommended Dilutions:\nELISA: \nCoat with 50-100ng Control Peptide/well\nAntibody Blocking:\nUse 5-10ug per 1ul of N0538-15 serum or 1ug of N0538-20 affinity purified.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

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