NCK1, Recombinant, Human (Cytoplasmic Protein Nck1, MGC12668, Nck1, Nck-1, Nck Adaptor Protein 1, SH2/SH3 Adaptor Protein NCK-alpha, SH2/SH3 Adaptor Protein NCK-alpha, NCK)

NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in signaling processes. Adaptor proteins usually contain several domains like SH2 (Src homology 2) and SH3 which allow specific interactions with other specific proteins. NCK1 and NCK2 showing high sequence identity (68%) have three SH3 domains and a C-terminal SH2 domain. Both of them bind receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling process of actin cytoskeleton reorganization have been identified. Ncks are thought to have important functions in the development of mesodermal structures during embryogenesis, linked to a role in cell movement and cytoskeletal reorganization. Nck also have a function in modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2 (eIF2). Under the stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2 through the phosphorylation, transiently inhibiting recycling of eIF2 into its active form.\n\nSource:\nRecombinant corresponding to aa1-377 with N-His tag, from human Nck1, expressed in E.coli.\n\nMolecular Weight:\n~45kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N0550-08C

Size

200ug

Form

Supplied as a lyophilized powder from 50mM NaH2PO4, pH8.0, 300mM sodium chloride, 170mM imidazole. Reconstitute with sterile dH2O PBS.

Purity

~95% (SDS-PAGE)

References

1. Latreille M and Larose L., 2006, J Biol Chem 281:26633-26644. 2. Frese S et al., 2006, J Biol Chem 281:18236-18245. 3. Bladt F et al., 2003, Mol Cell Biol 23:4586-4597.