NCX1 (Na+/Ca2+ Exchange Protein 1, CNC, DKFZp779F0871, MGC119581, Na+/Ca2+ Exchanger, NCX, Solute Carrier Family 8 Member 1, SLC8A1)

The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer,

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SPECIFICATIONS

Catalog Number

N0560-21

Size

100ul

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, GP, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

This product has been used in:Frank JS et al. Distribution of the Na(+)-Ca2+ exchange protein in mammalian cardiac myocytes: an immunofluorescence and immunocolloidal gold-labeling study. J Cell Biol 117:337-45 (1992). <PUBMED:1373142> Porzig H et al. Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies. Am J Physiol 265:C748-56 (1993). <PUBMED:7692739>Li ZP et al. The cardiac Na+-Ca2+ exchanger binds to the cytoskeletal protein ankyrin. J Biol Chem 268:11489-91 (1993). <PUBMED:8505285>Li Z et al. Expression of the cardiac Na(+)-Ca2+ exchanger in insect cells using a baculovirus vector. J Biol Chem 267:7828-33 (1992). <PUBMED:1560015>

Additional Info

Species Crossreactivity: Human, Rabbit, Rat, canine and Guinea pig.