NCX1 (Na+/Ca2+ Exchange Protein 1, CNC, DKFZp779F0871, MGC119581, Na+/Ca2+ Exchanger, NCX, Solute Carrier Family 8 Member 1, SLC8A1)

Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+:Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types (reviewed in 1-3). NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat brain, heart, kidney, lung, spleen, liver, or skeletal muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0560-21C

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a lyophilized powder in 5mM ammonium bicarbonate.Reconstitute with 50ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Hilgemann DW.2004. New insights into the molecular and cellular workings of the cardiac Na+/Ca2+ exchanger.Am J Physiol Cell Physiol. 287:C1167-C1172. 2. Canitano A, Papa M, Boscia F, Castaldo P, Sellitti S, Taglialatela M, Annunziato L. 2002. Brain distribution of the Na+/Ca2+ exchanger-encoding genes NCX1, NCX2, and NCX3 and their related proteins in the central nervous system.Ann N Y Acad Sci. 976:394-404. 3. Quednau BD, Nicoll DA, Philipson KD. 2004. The sodium/calcium exchanger family-SLC8. Pflugers Arch. 447:543-548. 4. Li XF, Lytton J. 1999. A cicularized sodium-calcium exchanger exon 2 transcript. J Biol Chem. 274:8153-8160. 5. Szerencsei RT, Tucker JE, Cooper CB, Winkfein RJ, Farrell PJ, Iatrou K, Schnetkamp PPM. 2000. Minimal domain requirement for cation transport by the potassium-dependent Na/Ca-K exchanger. 275:669-676. 6. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD. 2002. LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1. Nature Neurosci. 5:27 - 33. 7. Luo JH, Wang YH, Yasuda RP, Dunah AW, Wolfe BB. 1997. The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B). Mol. Pharmacol. 51:79 - 86. 8. Zukin RS and Bennett MVL. 1995. Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci., 18:306 - 313. 9. Laurie DJ. and Seeburg PH. 1994. Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14:3180 - 3194. 10. Foldes RL, Rampersad V, Kamboj RK. 1994. Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303 - 304.

Additional Info

Recognizes rat Sodium Calcium Exchanger 1. Species sequence homology: most mammals.