NCX1 (Sodium Calcium Exchanger)

Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. See catalog #N0560-20L: NCX1, Rat, Control Peptide (Sodium Calcium Exchanger).\nWestern Blot: 1-10ug/ml using ECL.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0560-20C

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Low, W., et al., FEBS Lett. 316: 63 (1993). 2. Furman, I., et al., FEBS Lett. 319: 105 (1993). 3. Lee, S.L., et al., J. Biol. Chem. 269: 14,849 (1994). 4. Nicoll, D.A., et al., J. Biol. Chem. 271: 24,914 (1996). 5. Kumoro, I., et al., PNAS 89: 4769 (1992). 6. Blaustein, M.P. & Lederer, J., Physiol. Rev. 79: 763

Additional Info

Recognizes rat NCX1.