NDE1 (Nuclear Distribution Protein nudE Homolog 1, NudE, NUDE, FLJ20101, HOM-TES-87)

NDE1 is important to microtubule reorganization, which is required for centrosome duplication and the formation of the mitotic spindle and cell migration. NDE1 is a binding partner of DISC1 along with LIS1 at the centrosome and at synapses in neurons. Mutations in the NDE1 gene result in a reduced cerebral cortex size caused by defects in mitotic progression and chromosomal localization of cortical progenitors. NDE1 is expressed as 2 isoforms produced by alternative splicing of the primary gene transcript.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse F0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138702

Size

50ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

~80% (SDS-PAGE)

Isotype

IgG1,k

References

1. Yan X., et al. (2003) Mol Cell Biol. 23(4):1239-50. 2. Kitagawa M., et al. (2000) FEBS Lett. 479(1-2):57-62. 3. Mayya V., et.al., Sci. Signal. 2:RA46-RA46(2009).

Additional Info

Recognizes human NDE1.