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NDUFS1 pAb

NDUFS1 pAb

Cat no: BS5818


Supplier: Bioworld Technology, Inc.
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The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1-7. NDUFS1, a 75kD protein, is the largest subunit of complex I, and is thought to be the first of the Fe-S proteins to accept electrons from an NADH-flavoprotein reductase within the complex. NDUFS1 may even form part of the active site crevice where NADH is oxidized. NDUFS1 is also a critical caspase substrate in mitochondria, and caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.
Catalogue number: BS5818
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P28331
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 662-707 of Human NDUFS1.
Species: NDUFS1 pAb detects endogenous levels of NDUFS1 protein.
Molecular weight: ~ 79 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; Complex I-75kD; CI-75kD; NDUFS-1
Additional info: For research use only, not for use in diagnostic procedure.

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