Necdin

Necdin is known to be a growth suppressor protein that is a member of the melanoma-associated antigen (MAGE) family and is mainly expressed in postmitotic neurons. Any expression of this protein ectopically halts growth. Necdin has been shown to play a role in modulating gene transcription. Nectin suppresses cell proliferation by interacting with hnRNP U. Necdin seems to be an important protein in the pathogenesis of Prader-Willi syndrome. Recently, Necdin has been linked to fetal and postnatal physiological myogenesis and regeneration of muscle.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml detects Necdin (~38kD) in NIH3T3 cells. NIH3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N0571. Proteins were visualized using a goat-anti rabbit IgG (HRP) and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH3T3 cells\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0571

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before glycerol was added to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Barker, P.A. and Salehi, A., J. Neurosci. Res. 67: 705-712 (2002). 2. Taniura, H. and Yoshikawa, K., J. Cell. Biochem. 84: 545-555 (2002). 3. Matsumoto, K., et al., Gene 272: 173-179 (2001). 4. Sciorati, C., et al., J. Cell. Sci. 122(8): 1119-1125 (2009). 5. Chapman, E.J. and Knowles, M.A., Mol. Carcinog. 48(11): 975-981 (2009).

Additional Info

Recognizes human Necdin at ~38kD. Non-specific proteins were also detected, Mr 70 and 100kD, detection of which were inhibited by preincubation with the immunizing peptide. Species Crossreactivity: mouse and rat