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Nedd 4, Recombinant, Human, N-terminal FLAG-Tag  (Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, KIAA0093, MGC176705, NEDD-4, NEDD4-1, Neural precursor cell expressed developmentally down-regulated protein 4, PIG53, RPF1)

Cat no: N0578

Nedd 4, Recombinant, Human, N-terminal FLAG-Tag (Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, KIAA0093, MGC176705, NEDD-4, NEDD4-1, Neural precursor cell expressed developmentally down-regulated protein 4, PIG53, RPF1)

Source:\nRecombinant corresponding to aa150-end of human Nedd 4 with N-terminal FLAG tag, expressed in a Baculovirus infected Sf9 cell expression system (NM_006154).\n\nSpecific Activity:\n0.0027pmol/min/ug \n\nAssay conditions: \n50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug UBE1), E2 (2.8ug UBCH5a), Biotin-Ubiquitin (0.5ug) and Nedd4 (0-2ug ) in 50mM Tris pH 7.4) incubate for 30 min at 37C, then add to streptavidin coated plates and incubate for 30 min at 37C. Add antibody against Nedd4, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at RT. (lambda-exc=330nm, lambda-em=620nm).\n\nApplications: \nSuitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening\ninhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

N0578

Size

20ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 80ug/ml FLAG peptide, 3mM DTT, 20% glycerol.

Purity

(same/more than)50% (SDS-PAGE)

References

1. Kotorashvili A. et al., J. Biol. Chem. 284(24), 16667-16678(2009). 2. Plant P.J. et al., Biochem. J. 419(1), 57-63(2009).

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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