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NEDD1 (Protein NEDD1, Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1, NEDD-1, FLJ35902, GCP-WD, TUBGCP7)

Cat no: 168385

NEDD1 (Protein NEDD1, Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1, NEDD-1, FLJ35902, GCP-WD, TUBGCP7)

Microtubules are polymers of tubulin, which exist as heterodimers of a-tubulin and b-tubulin. NEDD1(neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the g-tubulin ring complex (?-TuRC). ?-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the ?-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100,000\nWestern Blot: 1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

168385

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Ch/Bird

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Serum

Additional Info

Recognizes human NEDD1. Species sequence homology: Turkey, chicken, salmon, and danio (91%).

Alternative Names

GCP-WD, Neural precursor cell expressed developmentally down-regulated protein 1

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