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Nedd8, 1-76 aa, Recombinant, Human (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Prot

Cat no: N0580-02E

Nedd8, 1-76 aa, Recombinant, Human (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Prot

NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.\n\nEndotoxin Level: \n(same/less than) 1.0 EU per 1 ug of protein (determined by LAL method)\n\nMolecular Weight: 12.8kD (113 aa)\n\nAA Sequence:\nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE\nKTAADYKILG GSVLHLVLAL RGG\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N0580-02E

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 0.5mM DTT, 50mM sodium chloride, 10% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Wu K., et al. (2002) J Biol Chem. 277(1): 516-27 2. Hori T., et al. (1999) Oncogene. 18(48): 6829-34

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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