Neopterin, Human, BioAssay(TM) ELISA Kit

Sample Type:\nSerum, Plasma, Urine\n\nIntended Use:\nEnzyme immunoassay for the quantitative determination of neopterin in human serum, plasma and urine.\n\nTest Principle:\nSolid phase enzyme-linked immunosorbent assay (ELISA) based on the basic principle of a competitive ELISA. An unknown amount of antigen in the sample and a fixed amount of enzyme labelled antigen compete for the antibody-binding sites (rabbit-anti-neopterin). Both antigen-antibody complexes bind to the wells of the microtiter strips coated with a goat-anti-rabbit antibody. Unbound antigen is removed by washing. The intensity of the color developed after the substrate incubation is inversely proportional to the amount of antigen in the sample. Results of samples can be determined directly using the standard curve.\n\nKit Components:\nMicrotiter Plate, Coated with anti-rabbit IgG (goat, polyclonal), 1x96 wells\nStandard, 0nmol/L, 1x500ul\nStandard, 1.35nmol/L, 1x500ul\nStandard, 4nmol/L, 1x500ul, \nStandard, 12nmol/L, 1x500ul\nStandard, 37nmol/, 1x500ulL\nStandard, 111nmol/L, 1x500ul\nControl 1, 1x500ul\nControl 2, 1x500ul\nEnzyme Conjugate, Concentrate (201X), 1x0.1ml\nNeopterin Antiserum, 1x5ml\nTMB Substrate Solution, 1x17ml \nTMB Stop Solution, 1x17ml\nWash Buffer, Concentrate (20X), 1x50ml\nAssay Buffer, 1x18ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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