Nephrin (Y1210) (NPHS1, NPHN, Nephrin, Renal glomerulus-specific cell adhesion receptor) (FITC)

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

038894-FITC

Size

200ul

Applications

ELISA, FC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)\nWu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010)\nTossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010)\nMachuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)\nAya, K., et al. Kidney Int. 57(2):401-404(2000)

Additional Info

Human, mouse

Alternative Names

NPHS1, NPHN, Nephrin, Renal glomerulus-specific cell adhesion receptor