Nerve Growth Factor, beta, Recombinant, Human (Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb, HSAN5, MGC161426, MGC161428)

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.\n\nSource:\nDNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells.\n\nTheoretical Peptide Sequence: YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT\n\nBiological. Activity:\nED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: The NGF protein migrates at ~12-16kD in SDS-PAGE. It has a predicted molecular mass of 13.5kD. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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