Nerve Growth Factor, beta Recombinant, Human (NGFb)

Recombinant human nerve growth factor beta is a homodimer, glycosylated, polypeptide chain containing 2 identical 119aa and having a molecular mass of 16.9kD. The sequence of the first five N-terminal amino acids was found to be Ser-Ser-Ser-His-Pro.\n\nSource: \nHuman Nerve Growth Factor beta, from CHO cells.\n\nMolecular Weight:\n~16.9kD\n\nBiological Activity:\nThe ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be <1ng/ml, corresponding to a specific activity of >1x10e6Units/mg. \n\nEndotoxin: (same/less than)0.1ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-10B

Size

20ug

Form

Supplied as a lyophilized powder from 30mM sodium acetate, pH 5.5, 1% HSA. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml.

Purity

(same/more than)98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE).