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Nerve Growth Factor Receptor

Cat no: N2050-14A

Nerve Growth Factor Receptor

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunoprecipitation, Western Blot and Inhibition of NGF binding. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1-5ug/ml on rat\nWestern Blot: 1-5ug/ml (Non-reducing conditions only)\nImmunoprecipitation: 5ug/ml, 0.5% Triton X-100 buffer, PC12 positive control.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-14A

Size

50ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG1

References

1.

Additional Info

Specific for the rat low affinity Nerve Growth Factor-Receptor (p75).

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