Nerve Growth Factor Receptor, p75, Low Affinity (NGFR p75)

The low affinity NGFR (Nerve growth factor receptor) is a 75kD membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.\n\nApplications: \nSuitable for use in RIA, FACS, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Also suitable for the inhibition of NGF binding to p75NGFR. Reported by an independent laboratory to be suitable for ELISA. Other applications not tested.\n\nRecommended Dilution:\nFACS: Unfixed cells\nWestern Blot: 1ug/ml; RIPA lysates of human HS294T cells. Recognizes ~69-75kD band depending on gel conditions.\nImmunohistochemistry: 5-10ug/ml on frozen sections. Also suitable for formalin-fixed paraffin-embedded sections.\nImmunocytochemistry: 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-43

Size

100ug

Applications

FC, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Can, Fel, Prc, Rabbit, Sheep

Form

Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul sterile ddH2O.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Ross, A.H., et al., PNAS USA 81: 6681-6685 (1984). 2. Tremere, L.A., et al., Neuroreport 11: 2177-2183 (2000).

Additional Info

Recognizes the human low affinity nerve growth factor receptor (NGFR p75). The epitope recognized maps within the third or fourth cysteine-rich repeats of the NGFR molecule (within aa1-160, extracellular domain of human p75). Species Crossreactivity: primate, canine, feline, porcine, rabbit, raccoon and sheep. Does not react with rat, mouse or chicken.