$Nerve Growth Factor Receptor, p75\n(NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488)$

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Cat no: N2050-11N

Nerve Growth Factor Receptor, p75\n(NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488)

Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF, but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.\n\nProduced in P75 knock-out mouse.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry and NGF receptor p75 dynamics. Not appropriate for Western Blots. Does not block Neurotrophin binding. Other applications not tested. \n\nRecommended Dilution: \nImmunohistochemistry: 2ug/ml \nFlow Cytometry: 20ug/ml\nELISA: 1:5000 \nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for up to 4 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\nNote: We do not recommended adding glycerol as a cryopreservative.

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SPECIFICATIONS

Catalog Number

N2050-11N

Size

50ug

Applications

ELISA, FC, IF, IHC

Hosts

Mouse

Reactivities

Hum, Rat, Can, Fel, Prc, Rabbit, Sheep

Form

Supplied as a liquid in Tris, 50mM sodium chloride. Conjugated to ATTO 488.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2A

References

1.Walker BR et al. (2007) Behav Brain Res 176:109-20

Additional Info

Recognizes human p75NTR (low affinity neurotrophin receptor). Species Crossreactivity: rat, feline, canine, porcine, rabbit and sheep. Does not react with mouse.