$Nerve Growth Factor Receptor, p75\n(NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488)$

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Cat no: N2050-11R

Nerve Growth Factor Receptor, p75\n(NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488)

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry, Anti-NGF Receptor p75 Dynamics, Retrograde Transport Studies, and Study of Intracellular Trafficking. Not suitable for use in Western Blot or for blocking Neurotrophin binding. Other applications not tested. \n\nRecommended Dilution: \nImmunohistochemistry: 1-2ug/ml \nFlow Cytometry: 20ug/ml\nELISA: 1:5000\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for up to 4 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\nNote: We do not recommended adding glycerol as a cryopreservative.

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SPECIFICATIONS

Catalog Number

N2050-11R

Size

50ug

Applications

ELISA, FC, IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid. Conjugated to ATTO 488.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2A

References

1. Rogers ML, Atmosukarto I, Berhanu DA, Matusica D, Macardle P, Rush RA (2006). Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. J Neurosci Methods. 158(1) pp. 109-120. 2. Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. J. Neurosci. Res. 86(3) pp. 553-65. 3. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR. J. Neurosci. 28(6) pp. 1404-9. 4. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J. Neurosci. 27(30) pp. 7939-53.

Additional Info

Recognizes human p75NTR (low affinity neurotrophin receptor). Species Crossreactivity: mouse and rat. It is expected that this antibody will be useful for the study of p75 in primates and other species.