Nerve Growth Factor Receptor, p75NTR (Low-affinity, NGF receptor, Neurotrophin receptor, Gp80-LNGFR, p75 ICD)

Nerve Growth Factor Receptor, p75NTR, generated by Chandlers et al. p75NTR, was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. \n\nApplications:\nSuitable for use in ELISA, Western Blot, and Immunohistochemistry and immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1-2ug/ml stains paraformaldehyde-fixed, acetone-fixed and paraffin-embedded sections of rat spinal cord and brain. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-40A

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a lyophilized powder, 1% BSA. Reconstitute with 100ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Chandler, C. E. et al (1984) J Biol Chem 259:6882-6889. 2. Yan, Q., and Johnson, E. M., Jr. (1988) J Neurosci 8:3481-3498. 3. Huber, J., Dittrich, F., and Phelan, P. (1993) Eur J Biochem 218:1031-1039 \n4. Zhou, X. F., and Rush, R. A. (1996) J Comp Neurol 372:37-48. 5. Johnson, D. et al. (1986) Cell 47:545-54. 6. Tuffereau, C. et al (1998) Embo J 17:7250-9. 7. Kruger, G. M. et al. (2002) Neuron 35:657-69. 8. Stemple, D. L. & Anderson, D. J. (1992) Cell 71:973-85. 9. Urschel, B. A. & Hulsebosch, C. E. (1992) Brain Res Dev Brain Res 69:261-70. 10. Lagares A, et al. (2007) J Neurosci 27 (30):7939-7953.

Additional Info

Recognizes the extracellular domain of the neurotrophin receptor in p75NTR-expressing cells in rat spinal cord and brain. Does not cross react with p75NTR-expressing cells in other species.