Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Nestin is a class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.\nAlso, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. \n\nApplications: \nSuitable for use in Immunocytochemistry, Immunohistochemistry, ELISA and Western Blot. \nOther applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.\nImmunohistochemistry (frozen/paraffin): 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.\nWestern Blot: 1:1000-1:5000 using chemiluminescent detection. \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNestin transfected NS0 mouse myeloma cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2060-05B

Size

50ul

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Messam, C., J. Hou, and E.O. Major. Co-expression of nestin in neural and glial cells in the developing human CNS defined by a human specific anti-nestin antibody. Exp Neuro; Feb;161(2):585-96; 2000. 2. Messam C, Hou J, Berman JW, and Major EO. Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells. Developmental Brain Research. 134:87-92, 2002. 3. Attardo et al., Cerebral Cortex (2010) 20:304-314. \nGeneral references: 1. Hockfield, S., et al., J. Neurosci. 5:3310-3328 (1985). 2. Lendahl, U., et al., Cell 60:585-595 (1990). 3. Frederiksen, K., et al., J. Neurosci. 8:1144-1151 (1988). 4. Tohyama, T., et al., Lab. Invest. 66:303-313 (1992). 5. Uchida, N., et al., PNAS USA 97:14,720-14,725 (2000). 6. Frederiksen, K., et al., Neuron 1:439-448 (1988). 7. Cattaneo, C., et al., Nature 347:762-765 (1990). 8. Reynolds, B.A., et al., Science 255:1707-1739 (1992). 9. Rietze, R.L., et al., Nature 412:736-739 (2001). 10. Carpenter, M.K., et al., Exp. Neurol. 172:383-397 (2001). 11. Zulewski, H., et al., Diabetes 50:521-533 (2001). 12. Lumelsky, N., et al., Science 292:1389-1394 (2001). 13. Lechner, A., et al., Biochem. Biophys. Res. Commun. 293:670-674 (2002). 14. Shih, C.C., et al., Blood 98: 2412-2422 (2001).

Additional Info

Recognizes human nestin protein. Does not crossreact with rodent Nestin.