The patent pending, NEXTflex™ qRNA-Seq™ Kit, enables high precision gene expression analysis by RNA-Seq or ChIP-Seq.
Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. Quantitative ChIP-Seq applications begin with precipitated DNA at this step. Prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence.
Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels or copy number, irrespective of common amplification distortions observed in many RNA-Seq or ChIP-Seq experiments.
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