NIK (NF-kappa-beta-inducing Kinase, HsNIK, FTDCR1B, HS, Mitogen-activated Protein Kinase Kinase Kinase 14, MAP3K14, Serine/threonine Protein Kinase NIK)

NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK, initially isolated from a human B cell cDNA library, contains 795 amino acids with an apparent molecular weight of slightly more than 97kD on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nImmunoprecipitation: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2567-01A

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Malinin, N.L. et al., (1997) MAP3K-related kinase involved in NF-kB induction by TNF, CD95 and IL-1. Nature 385:540-544 2. Regnier, C.H., et al., (1997) Identification and characterization of an IkB kinase. Cell 90:373-383 3. Song, H.Y., et al., (1997) Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2. Proc. Natl. Acad. Sci. USA 94:9792-9796.

Additional Info

Reacts with human NIK.

Alternative Names

EC=2.7.11.25, NFkB-inducing Kinase