Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune responses, and apoptosis. Since most of the NO is oxidized to nitrite (NO2-) and nitrate (NO3-), the concentrations of these anions have been used as a quantitative measure of NO production. This nitric oxide fluorometric assay kit provides an accurate and convenient measurement of total nitrite/nitrate concentration in a simple two-step process. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step involves addition of DAN followed by NaOH, which converts nitrite into a fluorescent compound. Measurement of the fluorescence of this compound accurately determines the total nitric oxide production. The kit has been validated in culture media, plasma, and tissue homogenates. Some sample purification from these sources may be necessary.
Kit Components:
N2577-05A Assay Buffer 1x30ml
N2577-05B Enzyme Co-factors 2x1 vial
N2577-05C Nitrate Reductase 2x1 vial
N2577-05D Nitrate Standard 1x1 vial
N2577-05E DAN Reagent 1x1 vial
N2577-05F Sodium Hydroxide 1x1 vial
N2577-05G Microtiter Plates 2x96 wells
N2577-05H Plate covers 1x2 covers
N2577-05J Enhancer 1x1 vial
N2577-05K Nitrite Standard 1x1 vial
Storage and Stability:
All kit components are stable at -20 degrees C prior to reconstitution. After reconstitution, components are stable at 4 degrees C for no more than 24 hours.
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