Nod2

Nod2 is a member of the apoptosis regulating protein family that has caspase recruitment-domains (CARDs) and also includes Apaf-1 and Nod1.1 Nod2 contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). Nod2 is expressed in monocytes whereas Nod1 (CARD4) is expressed in multiple tissues. Both Nod1 and Nod2 act as intracellular receptors for bacterial lipopolysaccharides, activate NF-kB, and contribute to inflammatory bowel disease. A frameshift mutation in the NOD2 gene is associated with susceptibility to Crohn's disease, possibly by causing truncation of the tenth LRR.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-125 (may only be suitable for Nod2 transfected cells.).\nImmunohistochemistry: 2ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N3006-02

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide before the addition of glycerol to 40%

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB: J. Bertin, et al.; J. Biol. Chem. 274, 12955 (1999) \n2. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB: N. Inohara, et al. ; J. Biol. Chem. 274, 14560 (1999) \n3. An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways : N. Inohara, et al. ; J. Biol. Chem. 275, 27823 (2000) \n4. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB: Y. Ogura, et al.; J. Biol. Chem. 276, 4812 (2001) \n5. Induction of Nod2 in myelomonocytic and intestinal epithelial cells via nuclear factor-kappa B activation: O. Gutierrez, et al. ; J. Biol. Chem. 277, 41701 (2001) \n6. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease: Y. Ogura, et al. ; Nature 411, 603 (2001) \n7. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease: J.P. Hugot, et al. ; Nature 411, 599 (2001) \n8. Human Nod1 confers responsiveness to bacterial lipopolysaccharides : N. Inohara, et al. ; J. Biol. Chem. 276, 2551 (2001) \n9. Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis: Y. Ogura, et al. ; Gut 52, 1591 (2003)

Additional Info

Recognizes human Nod2 at 116kD..