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Notch1 (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

Cat no: N5375-07

Notch1 (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch 1, notch 2, notch 3, and notch 4 (Int3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of notch 1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch preproteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch 1 signaling or expression may contribute to tumorigenesis.\n\nApplications: \nSuitable for use in Immunohistochemistry, Western Blot and Flow Cytometry/FACS. Other applications not tested.\n\nRecommended Dilution: \nImmunohistochemistry (Formalin-fixed paraffin-embedded sections): 1:500 for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.\nWestern Blot: 1:500; Predicted MW: 290kD. A ~230kD band believed to be the processed extracellular fragment of Notch1 may also be detected.\nOptimal dilutions to be determined by the reseacher.\n\nPositive Control: \nImmunohistochemistry: Breast, colon or pancreas carcinoma.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-07

Size

100ul

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human Notch1. Crossreactivity with Notch 2 has not been tested. Species Crossreactivity: mouse.

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