NPTXR, Recombinant, Human, aa24-500, His-tag (Neuronal Pentraxin Receptor, NPR)

The Neuronal Pentraxin Receptor (NPTXR; also called NPR) is a 55-65kD, type II transmembrane glycoprotein within the Pentraxin family. NPTXR is co-expressed and forms heteromultimers with the related, secreted proteins NPTX1 and NPTX2/NARP. Human NPTXR is a 500 amino acid (aa) protein that includes a 477aa extracellular domain (ECD) with one calcium-binding Pentraxin domain. The human NPTXR ECD shares 88% and 89% aa sequence identity with mouse and rat NPTXR, respectively. A 62kD soluble form is generated by TACE/ADAM17 proteolytic cleavage C-terminal to the transmembrane segment. Soluble forms of 55kD and 45kD have also been reported. NPTXR is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. It is enriched on axonal membranes at excitatory synapses where, with NPTX1, it recruits the GluR4 subunit of AMPA-type glutamate receptors (AMPAR) to promote synaptogenesis. Synaptic activity stimulates mGluR1 or mGluR5 receptors, which then activate TACE, causing release of the NPTXR heteromultimer complex from the membrane. NPTX1/2 within the complex mediate binding AMPAR, while the soluble (but not transmembrane) form of NPTXR within the complex mediates AMPAR endocytosis and mGluR1/5-mediated long-term depression. NPTXR is reported to be increased in cerebrospinal fluid or serum of AlzheimerпїЅs disease patients as compared to controls. Aberrant expression of transmembrane NPTXR has also been reported in small-cell lung cancers.\n\nSource:\nRecombinant protein corresponding to aa24-500 from human Neuronal Pentraxin R/NPTXR, fused to his-tag at N-terminal, NS0-derived.\n\nMolecular Weight: \n~55-65kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

145826

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute in 300ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Dodds, D.C. et al. (1997) J. Biol. Chem. 272:21488. 2. Kirkpatrick, L.L. et al. (2000) J. Biol. Chem. 275:17786. 3. Cho, R.W. et al. (2008) Neuron 57:858. 4. Sia, G.M. et al. (2007) Neuron 55:87. 5. Yin, G.N. et al. (2009) Brain Res. 1265:158. 6. Poulsen, T.T. et al. (2005) Lung Cancer 50:329.