Nucleic Acid Detection Kit, Biotinylated, BioAssay(TM)

Nucleic Acid Detection Kit, Biotinylated, BioAssay(TM) for detecting biotinylated nucleic acid probes using Alkaline Phosphatase Streptavidin and the Chemiluminescent/Fluorescent Substrate. One kit contains sufficient reagents to develop approximately 20 x 100cm2 blots. Kit is optimized to reproducibly provide high sensitivity with low background in applications such as Southern or northern hybridization, dot blotting, or plaque or colony screening.\n\nBiotin can be incorporated into a nucleic acid probe using various enzymatic or non-enzymatic methods. Biotinylated probes are recognized by streptavidin coupled to alkaline phosphatase. The probe is ultimately visualized by the conversion of Chemiluminescent/Fluorescent Substrate to a luminescent and fluorescent product by a dephosphorylation reaction catalyzed by alkaline phosphatase. The sensitivity is enhanced with the use of the Blocking Reagent and wash buffers included in the kit that are specifically designed to minimize background. Chemiluminescence can be documented by exposure to film or by image capture with digital imaging systems. Blots can be exposed to film several times over an extended period of time, with typical film exposure times of 30 seconds to 10 minutes. \n\nSensitivities as low as 100fg of target nucleic acid can be achieved. Optimal exposure times using digital imaging systems depend on the sensitivity of the instruments. Chemiluminescence can be developed on either nylon or nitrocellulose membranes, although nylon is preferred because of faster signal production. The fluorescence of the developed substrate can be recorded with a digital imaging system or conventional camera months after the chemiluminescence has faded. Acquiring an image from the fluorescent signal requires a much shorter exposure time than from the chemiluminescent signal, often just a fraction of a second.\n\nKit Components:\nChemiluminescent/Fluorescent Substrate 1x100ml\nAlkaline Phosphatase Streptavidin 1x600ul\n10X Blocking reagent 1x120ml\n20X wash A 1x120ml\n10X wash B 1x120ml\n\nStorage and Stability:\nStore powder at 4 degrees C, liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N6927-70

Size

1Kit

References

1. Vidakovic M, Gluch A, Qiao J, Oumard A, Frisch M, Poznanovic G, Bode J. 2009. PARP-1 expression in the mouse is controlled by an autoregulatory loop: PARP-1 binding to an upsteam SA/MAR element and to a novel recognition motif in its promoter suppressestranscription. JMolBiol.288:730-50. 2. Qian Y, Venkatraj J, Barhoumi R Pal R, Datta A, Wild JR, Tiffany-Castiglioni E. 2007. Comparative non-cholinergic neurotoxic effects of paraoxon and diisopropyl fluorophosphates (DFP) on human neuroblastoma and astrocytoma cell lines. Toxicol Appl Pharmacol. 219(2-3):162-71. Wu WWH, Sun Y-HB, Pante N. 2007. 3. Nuclear import of influenza A viral ribonucleoprotein complexes is mediated by two nuclear localization sequences on viral nucloprotein. Virology J. 4:49-60. Lonskaya, I., 4. Potaman, V.N., Shlyakhtenko, L.S., Oussatcheva, E.A., Lyubchenko, Y.L., Soldatenkov, V.A. 2005. Regulation of poly(ADP-ribose) polymerase-1 by DNA structure-specific binding. J Biol Chem. 280(17):17076-83. 5. Johnson, C.D., Balagurunathan, Y., Tadesse, M.G., Falahatpisheh, M.H., Brun, M., Walker, M.K., Dougherty, E.R., Ramos, K.S. 2004. Unraveling gene