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Nucleoside Diphosphate Kinase A, aa1-152, Recombinant, Human (NME1, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1)

Cat no: N7100-67

Nucleoside Diphosphate Kinase A, aa1-152, Recombinant, Human (NME1, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1)

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 , expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant human NME1 (aa1-152) expressed in E. coli (NP_000260).\n\nAA Sequence:\nMANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N7100-67

Size

100ug

Form

Supplied as liquid iIn 20mM Tris-HCl buffer, pH7.5, 1mM DTT, 10% glycerol

Purity

~95% by SDS-PAGE Purified by immunoaffinity chromatography.

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