BAP31 (CT) (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E)

Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress- induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

B0077-02B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Ng, F., et al., (1997), \"p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein in the endoplasmic reticulum\", J. Cell Biol., 139: 327-338. 2. Kim, K.M., et al., (1994), \"Two new proteins preferentially associated with membrane immunoglobulin D\", EMBO J., 13: 3793-3800. 3. Paquet, M-E., et al., (2004), \"Bap29/31 influences the intracellular traffic of MHC class I molecules\", J. Immunol., 172: 7548-7555. 4. Zen K., et al., (2004), \"Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils\", J. Biol. Chem., 279: 44924-44930. 5. Nguyen, M., et al., (2000), \"Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria\", Mol. Cell. Biol., 20: 6731-6740.

Additional Info

Recognizes human BAP31, C-terminal. Species Crossreactivity: mouse, rat.