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BAP31 (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E)

Cat no: B0077-03

BAP31 (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E)

BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis (1,2). During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria (2-4). Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway (2,5). Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface (6-10).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0077-03

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by peptide affinity chromatography.\n

Isotype

IgG

References

(1) Adachi, T. et al. (1996) EMBO J 15, 1534

Additional Info

Detects endogenous levels of total BAP31 protein.

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