Nur77/NR4A1 (G347) pAb

Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.

Prices direct from Bioworld Technology, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

BS3260

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 320-365 of Human Nur77/NR4A1.

Species

Nur77/NR4A1 (G347) pAb detects endogenous levels of Nur77/NR4A1 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P22736

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul