OAT 4 (Organic anion transporter 4)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Most recently, a novel member of the OAT family, OAT4, has been cloned and characterized. OAT4 (human 550 aa) is ~38-44% identical to other OATs. It is prominently expressed in the kidney and placenta. OAT4 interacted with chemically heterogeneous anionic compounds (nonsteroidal antiinflammatory drugs, diuretics, sulfobromopthalein, penicillin G, and bile acid salts). OAT4 may help in eliminating harmful anionic substances and protect the fetus.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. O3003-30. Control peptide can be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1-10ug/ml using ECL. Kidney or placenta can be used as positive control.\nImmunohistochemistry: 2-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O3003-32

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zhou F, 2004, Mol. Pharmacol., 65:1141-1147, WB 2. Ugele B, 2003, Am J Physiol. Endocrinology Metab: 284, 390-398. IHC 3. Cha SH et al (2000) J. Biol. Chem. 275, 4507-4512.

Additional Info

Recognizes human OAT4. No significant sequence homology is detected with other OATs or other proteins.