Olfactomedin-2, Recombinant, Human (OLFM2, OM2, neuronal olfactomedin-related endoplasmic reticulum- localized-2, Noelin-2)

Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum- localized-2 (Noelin-2) i s a 50kD (predicted), secreted member of the olfactomedin/noelin family of proteins. Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name. Olfactomedin domain-containing proteins are of ten found in neural tissues. Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum. In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation. The human Olfactomedin-2 cDNA encodes a 20aa signal sequence and a 454 mature protein that contains two coiled-coil regions (aa58-85 and 136-193) and an Olfactomedin domain (aa194-446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus , and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2. Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients. The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noel in-1 might heterodimerize with Olfactomedin-2. Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication. \n\nSource:\nChinese Hamster Ovary cell line, CHO-derived. Gln21-Pro454, with a C-terminal 6-His tag.\n\nMolecular Weight: 55-75kD (SDA-PAGE, reducing conditions) \n\nBiological Activity:\nMeasured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells. When 5x10er cells/well are added to human Olfactomedin-2 coated plates (1ug/ml, 100ul/well), ~50-75% will adhere after 1h at 37 degrees C. \n\nEndotoxin: (same/less than)1EU/ug (LAL) \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with 100ug/ml PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O6170-02

Size

50ug

Form

Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 100ug/ml PBS.

Purity

(same/more than)90% (SDS-PAGE)

References

1. Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304. 2. Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41. 3. Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122. 4. Moreno, T.A. & M. Bronner