Omi, HtrA2, aa134-458, Recombinant, Human

HtrA2/Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death. Mature form of HtrA2/Omi (residues 134-458) was overexpressed in E. coli.\n\nSequences:.\nMAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O6350-15

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM DTT and 20% glycerol.

Purity

> 95% by SDS-PAGE. Purified by using conventional chromatography techniques.

References

1. Savopoulos, J.W., et al., Protein Expr. Purif. 19: 227-234 (2000). 2. Gray, C.W., et al., Eur. J. Biochem. 267: 5699-5710 (2000). 3. Martins, L.M., et al., J. Biol. Chem. 277: 439-444 (2002). 4. Van Loo, G., et al., (2002) Cell Death Differ. 9: 20-60 (2002). 5. Seong, Y.M., et al., Protein Expr. Purif. 33: 200-208 (2004).