Oncostatin M, Recombinant, Rat (OSM)

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis.Produced by activated T cells, monocytes and Kaposirsquos sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation.It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposirsquos sarcoma cells, but, inhibits the growth of some normal and tumor cell lines.It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells.OSM share several structural and functional characteristics with LIF, IL-6, and CNTF.Human OSM is active on murine cells.Recombinant rat Oncostatin M is a 24.4kD protein, containing 215 amino acid residues.\n\nBiological Activity:\nAssay #1: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0ug/ml.\nAssay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.\n\nAA Sequence:\nMKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts