Osteocrin, Recombinant, Human

Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.\n\nDescription: \nThe recombinant Human Osteocrin is produced with N-terminal fusion of HisTag. The Human Osteocrin His-Tagged Fusion Protein is 13.6kD containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues

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SPECIFICATIONS

Catalog Number

O8061-18

Size

2ug

Form

Supplied as a lyophilized powder in 5mM Tris, 25mM sodium chloride, pH 7.5.

Purity

(same/more than) 95% (SDS-PAGE); One-step procedure using affinity Ni-NTA chromatography.

References

1. Characterization of osteocrin expression in human bone.\n\nJ Histochem Cytochem 2005 Oct;53(10):1181-7\n\n2. Osteocrin, a novel bone-specific secreted protein that modulates the osteoblast phenotype.\n\nJ Biol Chem 2003 Dec 12;278(50):50563-71