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Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1)

Cat no: O8064-06

Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1)

Osteopontin is a single-chain polypeptide with a molecular weight of ~35kD. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000\nImmunohistochemistry (Paraffin): 1:100-1:1500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O8064-06

Size

20ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Reconstitute with 20ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes human osteopontin. Does not crossreact with human osteonectin or bone sialoprotein.

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