Home  >  Products  >  OTUB1 (Ubiquitin Thioesterase OTUB1, Deubiquitinating Enzyme OTUB1, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing Protease OTUB1, OTB1, OTU1, HSPC263)
OTUB1 (Ubiquitin Thioesterase OTUB1, Deubiquitinating Enzyme OTUB1, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing Protease OTUB1, OTB1, OTU1, HSPC263)

OTUB1 (Ubiquitin Thioesterase OTUB1, Deubiquitinating Enzyme OTUB1, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing Protease OTUB1, OTB1, OTU1, HSPC263)

Cat no: O8490-01B

Supplier: United States Biological
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Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.03-0.1ug/ml, observed in human brain (cerebellum) and mouse and rat brain lysates on ~30kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: O8490-01B
Reactivities: Human, Mouse, Rat, Bovine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Juris SJ, Shah K, Shokat K, Dixon JE, Vacratsis PO. Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. FEBS Lett. 2006 Jan 9;580(1):179-83.
Additional info: Recognizes mouse OTUB1. Species sequence homology: Bovine, human and rat.

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