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p-4E-BP1 (T46) pAb

p-4E-BP1 (T46) pAb

Cat no: BS4747


Supplier: Bioworld Technology, Inc.
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The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
Catalogue number: BS4747
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: Q13541
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 46.
Species: p-4E-BP1 (T46) pAb detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr46.
Molecular weight: ~ 15 to 20 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1; 4EBP1; PHAS I; PHASI;
Additional info: For research use only, not for use in diagnostic procedure.

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