p-C/EBP-(beta) (T235/188) pAb

C/EBP (beta) is a member of the C/EBP transcription factor family. The C/EBP (beta) gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP (beta) isoforms. C/EBP (beta) is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP (beta) contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP (beta) gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP (beta) form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP (beta) isoforms during acute-phase reaction occurs through its upregulation and structural modification.

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SPECIFICATIONS

Catalog Number

BS4029

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human C/EBP-(beta) around the phosphorylation site of Threonine 235.

Species

p-C/EBP-(beta) (T235/188) pAb detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P17676

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 19, 36, 38 kDa

Alternative Names

CCAAT/enhancer-binding protein beta; C/EBP (beta); C/EBP(beta); C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; TCF5; ORF Names: PP9092