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p-Cdc25B (S323) pAb

p-Cdc25B (S323) pAb

Cat no: BS4034


Supplier: Bioworld Technology, Inc.
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The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.
Catalogue number: BS4034
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: P30305
Dilutions: WB: 1:500~1:1000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Serine 323.
Species: p-Cdc25B (S323)pAb detects endogenous levels of Cdc25B protein only when phosphorylated at Ser323
Molecular weight: ~ 65 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Additional info: For research use only, not for use in diagnostic procedure.

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