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p-Cofilin (S3) pAb

p-Cofilin (S3) pAb

Cat no: BS4716


Supplier: Bioworld Technology, Inc.
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Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.
Catalogue number: BS4716
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P23528
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.
Species: p-Cofilin (S3) pAb detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.
Molecular weight: ~ 19 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL
Additional info: For research use only, not for use in diagnostic procedure.

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