p-ER(alpha) (Y537) pAb

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER(alpha) and ER(beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER(alpha) and ER(beta) have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER(alpha) and ER(beta) may be regulated by distinct mechanisms even though they share many functional characteristics.

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SPECIFICATIONS

Catalog Number

BS4683

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human ER(alpha) around the phosphorylation site of Tyrosine 537.

Species

p-ER(alpha) (Y537) pAb detects endogenous levels of ER(alpha) protein only when phosphorylated at Tyr537.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P03372

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul