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p-Histone H2A.X (S139) pAb

Cat no: BS4760

p-Histone H2A.X (S139) pAb

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

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SPECIFICATIONS

Catalog Number

BS4760

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Species

p-Histone H2A.X (S139) pAb detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P16104

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 15 kDa

Alternative Names

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; (gamma)-H2AX; (gamma)H2AX; ganunaH2AX; (gamma)-H2A.X; (gamma)H2A.X; ganunaH2A.X

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