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p-Histone H3 (T11) pAb

Cat no: BS4699

p-Histone H3 (T11) pAb

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions.

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SPECIFICATIONS

Catalog Number

BS4699

Size

100ug/100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Threonine 11.

Species

p-Histone H3 (T11) pAb detects endogenous levels of Histone H3 protein only when phosphorylated at Thr11. (removal of the initiator methionine)

Dilutions

WB: 1:500~1:1000\nIF: 1:50~1:200

Swiss Prot

P68431/Q71DI3/P84243

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 17 kDa

Alternative Names

Histone H3.1; H3/a; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l;

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